Journal: Nature Communications

Article Title: Sleep fMRI with simultaneous electrophysiology at 9.4 T in male mice

doi: 10.1038/s41467-023-37352-9

Figure Lengend Snippet: a Design and location of MR-compatible ECoG array. The array was placed above the dura and centered on bregma (length, ~4 mm; width, ~6 mm) for electrocorticogram (ECoG) recording with two gold wires (orange) inserted in nuchal muscles for electromyography (EMG) recording. Right panel, the diagram of multi-electrode ECoG array and its layered construction. FPC, flexible printed circuit. b Design and location of depth electrode. Graphene fiber (GF) based MR-compatible depth electrode was implanted into the dorsal CA1 region of the left hippocampus with gold wires (orange) placed on dura and nuchal muscles for medial prefrontal cortex (mPFC) intracranial electroencephalography (iEEG) and EMG recordings, respectively. c The pipeline of animal surgery, recovery, habituation training and mouse sleep fMRI data acquisition with details described in Method. The cartoon mouse image was drawn by Figdraw. d Illustration of field of view (FOV) during simultaneously electrophysiological and fMRI recording. e Minimal MRI artifacts of ECoG arrays (upper) or GF depth electrodes (lower) on T2 weighted structural (left panel) and T2* weighted functional images (right panel) of the mouse brain. f Schematic illustration of the mouse sleep fMRI setup with details described in Method.

Article Snippet: The array was made up of a flexible printed circuit (FPC) and two insulated gold wires, all soldered to an Omnetics 18-pin MR-compatible connector (A70242-001, Omnetics, USA, Minneapolis).

Techniques: Muscles, Functional Assay